Interaction with a wild-type ovule
Video 11. Pollen tube interaction with a wild-type ovule in an in vitro guidance assay
Time-lapse imaging of LAT52:DsRed -tagged pollen tube entering a wild-type ovule and traveling within the ovule before arresting growth and bursting in the DD3:GFP -marked synergid cell.
The targeted ovule repels the second, late-arriving pollen tube away from the ovule micropyle.
The video shows merged time-lapse images captured in red and green channels.
Time elapsed between two frames is 10 minutes.
Ref: Tsukamoto, T., Qin Y., Huang, Y., Dunatunga, D. and Palanivelu, R. (2010). Plant J (in press).
(Click image to play video)
