Interaction with a wild-type ovule

Video 11. Pollen tube interaction with a wild-type ovule in an  in vitro  guidance assay

Time-lapse imaging of  LAT52:DsRed -tagged pollen tube entering a wild-type ovule and traveling within the ovule before arresting growth and bursting in the  DD3:GFP -marked synergid cell.

The targeted ovule repels the second, late-arriving pollen tube away from the ovule micropyle.

The video shows merged time-lapse images captured in red and green channels.

Time elapsed between two frames is 10 minutes.

Ref:  Tsukamoto, T., Qin Y., Huang, Y., Dunatunga, D. and Palanivelu, R. (2010). Plant J (in press).

(Click image to play video)