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Interaction with synergid cell III

  Video 9. Pollen tube interaction with the synergid cell in an  in vitro  guidance assay

Time-lapse imaging of DsRed-tagged pollen tubes entering the ovule and traveling within the ovule before approaching the GFP-tagged synergid cell within the female gametophyte.

Left video, time-lapse images captured in the red and green channels. Right video, time-lapse images captured in the green channel only.

The time elapsed between frames is 10 minutes (indicated on the top left hand corner of each video),

After arriving at the synergid cell, the pollen tube continues to elongate near the synergid cell. In this example, the pollen tube grows perpendicular to the synergid cell, extending one (110 minutes frame) or two times (190 minutes frame) around the synergid cell.

Ref:  Nikolova, N*., Palanivelu, R*., King, E., Copenhaver, G., and Drews, G. N. (2007). Plant Physiology, 144:1753-1762 .

(Click image to play video)

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Video | by Dr. Radut