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Guidance assay

Video 1.  In vitro  pollen tube guidance assay  monitors ovule targeting by pollen tubes.

Time-lapse imaging of GFP-tagged pollen tubes emerging from the cut portion of the pistil, travelling across the agarose medium before approaching a subset of excised ovules.

In this instance, pollen tubes grew within 100 µm only near two of the six ovules; of these two, only one was successfully targeted.

Ref:  Palanivelu & Preuss (2006), BMC Plant Biology, 6:7 .

(Click image to play video)

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Video | by Dr. Radut