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Glutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine.

TitleGlutamate receptor-like genes form Ca2+ channels in pollen tubes and are regulated by pistil D-serine.
Publication TypeJournal Article
Year of Publication2011
AuthorsMichard E, Lima PT, Borges F, Silva ACatarina, Portes MTeresa, Carvalho JE, Gilliham M, Liu L-H, Obermeyer G, Feijó JA
Date Published2011 Apr 22
Keywords6-Cyano-7-nitroquinoxaline-2,3-dione, Arabidopsis, Calcium, Calcium Channels, Calcium Signaling, Cell Membrane, Cytosol, Excitatory Amino Acid Agonists, Excitatory Amino Acid Antagonists, Flowers, Gene Expression Regulation, Plant, Genes, Plant, Glycine, Morphogenesis, Patch-Clamp Techniques, Plants, Genetically Modified, Pollen Tube, Racemases and Epimerases, Receptors, Glutamate, Serine, Tobacco

Elevations in cytosolic free calcium concentration ([Ca(2+)](cyt)) constitute a fundamental signal transduction mechanism in eukaryotic cells, but the molecular identity of Ca(2+) channels initiating this signal in plants is still under debate. Here, we show by pharmacology and loss-of-function mutants that in tobacco and Arabidopsis, glutamate receptor-like channels (GLRs) facilitate Ca(2+) influx across the plasma membrane, modulate apical [Ca(2+)](cyt) gradient, and consequently affect pollen tube growth and morphogenesis. Additionally, wild-type pollen tubes grown in pistils of knock-out mutants for serine-racemase (SR1) displayed growth defects consistent with a decrease in GLR activity. Our findings reveal a novel plant signaling mechanism between male gametophyte and pistil tissue similar to amino acid-mediated communication commonly observed in animal nervous systems.

Alternate JournalScience
PubMed ID21415319

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