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Protocols

Lab Manual

Pollen Course Lab Manual
Description: The lab manual used for the Methods in Pollen Research Course.
Download Protocol: RCN Contributor: Pollen RCN

Microscopy

Auramine of staining of pollen exine
Description: Auramine of staining of pollen exine description
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Reference: PubMed
RCN Contributor: Pollen RCN

Aniline blue staining of pollen tube in the Arabidopsis pistil
Description: Aniline blue staining of pollen tube in the Arabidopsis pistil description
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Reference: PubMed
RCN Contributor: Pollen RCN

Congo red staining of pollen tube growth in Arabidopsis pistils
Description: Congo red staining of pollen tube growth in Arabidopsis pistils description.
Download Protocol: RCN Contributor: Pollen RCN

Miscellaneous

GUS staining protocol
Description: GUS staining protocol
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Reference: PubMed
RCN Contributor: Pollen RCN

Pollen Tube Growth

Growing Arabidopsis pollen tubes in vitro
Description: Growing Arabidopsis pollen tubes in vitro description.
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Reference: PubMed
RCN Contributor: Pollen RCN

Growing Arabidopsis pollen tubes by the semi in vivo (SIV) method
Description: Video Illustration of the Protocol (Hosted at the Palanivelu lab Web site)
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Reference: PubMed
RCN Contributor: Anonymous

RNA

Arabidopsis pollen RNA extraction
Description: Arabidopsis pollen RNA extraction description
Download Protocol: RCN Contributor: Pollen RCN

Undergraduate Teaching

Comparative flower anatomy, fluorescent staining, and in vitro pollen germination
Description: This lab was developed for undergraduates in a 200-level (sophomore) course titled "Developmental Biology" and assumes little knowledge of flower form or function.
Download Protocol: RCN Contributor: edlunda

Boron requirements in pollen tube elongation and hand crossing in tobacco
Description: This lab was developed for undergraduates as part of an introductory course titled "The Plant Organism."
Download Protocol: RCN Contributor: mark_johnson