Protocols
Lab Manual
Description: The lab manual used for the Methods in Pollen Research Course.
Download Protocol:
Pollen-Course-Methods-2011.pdf

Microscopy
Description: Auramine of staining of pollen exine description
Reference: PubMed
Download Protocol:
Auramine_O_Dobritsa.doc

Description: Aniline blue staining of pollen tube in the Arabidopsis pistil description
Download Protocol:
Aniline_blue_Toshiyuki.pdf

Description: Congo red staining of pollen tube growth in Arabidopsis pistils description.
Download Protocol:
Congo_red_staining_pistils.pdf

Miscellaneous
Pollen Tube Growth
Description: Growing Arabidopsis pollen tubes in vitro description.
Download Protocol:
Protocol_for_Arabidopsis_Pollen_in vitro.doc

Description: Video Illustration of the Protocol (Hosted at the Palanivelu lab Web site)
Download Protocol:
Protocol_for_Arabidopsis_Pollen_SIV.doc

RNA
Description: Arabidopsis pollen RNA extraction description
Download Protocol:
Arabidopsis pollen RNA extraction.pdf

Undergraduate Teaching
Description: This lab was developed for undergraduates in a 200-level (sophomore) course titled "Developmental Biology" and assumes little knowledge of flower form or function.
Download Protocol:
Plant Lab Dev Bio 2011_5.pdf

Description: This lab was developed for undergraduates as part of an introductory course titled "The Plant Organism."
Download Protocol:
pollination-and-fertilization.doc
