Pollen tube development.
|Title||Pollen tube development.|
|Publication Type||Journal Article|
|Year of Publication||2010|
|Authors||Johnson MA, Kost B|
|Journal||Methods Mol Biol|
|Keywords||Arabidopsis, Cell Culture Techniques, Germination, Mutation, Plant Proteins, Pollen Tube, Tobacco, Transformation, Genetic|
Pollen tubes grow rapidly in a strictly polarized manner as they transport male reproductive cells through female flower tissues to bring about fertilization. Vegetative pollen tube cells are an excellent model system to investigate processes underlying directional cell expansion. In this chapter, we describe materials and methods required for (1) the identification of novel factors essential for polarized cell growth through the isolation and analysis of Arabidopsis mutants with defects in pollen tube growth and (2) the detailed functional characterization of pollen tube proteins based on transient transformation and microscopic analysis of cultured tobacco pollen tubes.
|Alternate Journal||Methods Mol. Biol.|
|Grant List||/ / Biotechnology and Biological Sciences Research Council / United Kingdom|